Journal of Medicinal Materials, 2025, Vol. 30, No. 2 (pp. 125 - 131)

α-AMYLASE AND α-GLUCOSIDASE INHIBITORY ACTIVITIES
OF THE FLOWER EXTRACTS FROM OCHNA INTEGERRIMA

Nguyen Phuc Linh Gabriel¹, Bui Minh Phuc², Nguyen Ngoc Hoang Nhi¹,
Ton Nu Lien Huong², Nguyen Minh Hien^1,*

¹Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam;

²College of Natural Sciences, Can Tho University, Can Tho City, Vietnam

*Corresponding author: nguyenminhhien@tdtu.edu.vn

Summary

α-Amylase and α-Glucosidase Inhibitory Activities of the Flower Extracts from Ochna integerrima

This research aimed to examine the inhibitory effects of n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc), aqueous, and methanol (MeOH) extracts of Ochna integerrima on α-amylase and α-glucosidase enzymes for the first time. Out of the five extracts tested, the aqueous extract exhibited remarkable anti-glucosidase and anti-amylase activities with IC₅₀ values of 8.78 ± 0.26 and 7.16 ± 0.13 mg/mL, respectively, followed by n-hexane extract with IC₅₀ values of 71.82 ± 1.43 and 42.45 ± 0.69 mg/mL. Then, compounds were isolated from the active extracts. Stigmasterol (1) and maslinic acid (2), extracted from n-hexane, exhibited inhibitory effects against α-amylase with IC₅₀ values of 71.65 ± 1.34 μM and 48.06 ± 1.6 μM, respectively. However, the isolated compounds 6-(γ,γ-dimethylallyl)taxifolin 7-O-β-D-glucoside (3) and dimethylallyl-dihydrokaempferol 7-O-β-D-glucoside (4) from aqueous extract did not support the observed effect. Further investigation of the bioactive compounds from the aqueous extract is necessary to support its potent activity.

Keywords: α-Amylase; α-Glucosidase; Extracts; Ochna integerrima flowers.

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