Journal of Medicinal Materials, 2025, Vol. 30, No. 6 (446 - 454)

IN VITRO AND IN VIVO EVALUATION OF ANTI-INFLAMMATORY

AND ANALGESIC EFFECTS OF GYNURA PROCUMBENS (LOUR.)
MERR. LEAF EXTRACT

Ngo Kien Duc^1,2,*, Pham Diem Thu¹, Thai Le Duan¹, Tran Thanh Nhan¹

¹School of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam;

²Department of Biochemistry, Thong Nhat Hospital, Ho Chi Minh City, Vietnam

*Corresponding author: ngokienduc@ump.edu.vn

Summary

This study evaluated the anti-inflammatory and analgesic effects of a 95% ethanol extract of Gynura procumbens leaves. These effects were assessed using both in vitro and in vivo models. In vitro anti-inflammatory activity was assessed using the egg white albumin denaturation assay, while in vivo efficacy was evaluated via xylene-induced ear edema and hot plate models in mice at doses of 37.5, 50, and 75 mg/mL. Chemical analysis revealed 22.3 mg essential oil/kg of extract and 22.44 mg quercetin equivalents/g of extract. It inhibited albumin denaturation with an IC₅₀ of 0.34 mg/mL, significantly lower than the IC₅₀ of diclofenac. In the xylene-induced ear edema model, all doses reduced ear thickness, with the 75 mg/mL dose exhibiting an effect comparable to diclofenac. In the hot plate test, the 50 and 75 mg/mL doses significantly increased response latency. These findings demonstrate that the ethanol extract of G. procumbens leaves possesses significant anti-inflammatory and analgesic properties, supporting its potential as a natural therapeutic candidate for inflammation and pain management.

Keywords: Gynura procumbens; Anti-inflammatory; Analgesic.

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