Journal of Medicinal Materials, 2025, Vol. 30, No.3 (pp. 172 - 178)

METHOD DEVELOPMENT FOR SIMULTANEOUS QUANTIFICATION

OF NARINGIN AND MELITIDINE IN YOUNG POMELO FRUITS

BY HPLC-PDA

Nguyen Thi Anh Nguyet^1,*, Nguyen Kieu Quynh Trang¹, Nguyen Tan Phat¹, Nguyen Hoai Sang¹,  Nguyen Le Hoang Son², Hoang Ngoc Cuong³, Huynh Loi², Tran Thi Van Anh¹, Le Van Huan²

¹University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam;

²Institute of Pharmaceutical Education and Research, Binh Duong University, Vietnam;

³Faculty of Food Technology, Binh Duong University, Vietnam.

*Corresponding author: ntanguyet@ump.edu.vn

Summary

In this study, an HPLC method was developed for quantitative analysis of melitidine and naringin, which were found in young pomelo fruits. This procedure was performed on the Waters Acquity UPLC system, using a GL Sciences InertSustain C18 chromatographic column (4.6 × 250 mm; 5 µm). Mobile phase (acetonitrile - acetic acid 0.1%), flow rate of 1 mL/min, and injection volume of 10 µL, UV detection at 283 nm. The developed method met all validation requirements of ICH guidelines as follows: System suitability, specificity, and linear range of naringin (50 - 1000 µg/mL) and melitidine (25 - 500 µg/mL) with R² = 0.9999. The limits of detection (LODs) and limits of quantification (LOQs) of naringin and melitidine at 0.17 µg/mL and 0.56 µg/mL, respectively; the intraday precision (RSD = 0.45 - 1.68%) and interday precision (RSD = 0.61-1.92%); the accuracy from 95.08% to 102.94% (RSD = 0.55 - 1.82%). This method was applied for the analysis of naringin and melitidine in some samples of young pomelo fruit collected in Binh Duong.

Keywords: Citrus maxima; Young pomelo; Naringin; Melitidin: HPLC.

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