Journal of Medicinal Materials, 2025, Vol. 30, No. 5 (368 - 374)
MICROSCOPIC CHARACTERISTICS, HPTLC PROFILING, AND EVALUATION OF ANTIOXIDANT, TYROSINASE INHIBITORY,
AND ANTI-INFLAMMATORY ACTIVITIES OF EURYA NITIDA KORTH. LEAF EXTRACTS
Nguyen Phuc Linh Gabriel1, Nguyen Minh Tien1, Ngo The Kieu Trang1, Nguyen Ngoc Hoang Nhi1, Huynh Ha Thy1, Nguyen Phuc Khanh1,
Vu Huynh Kim Long2, Nguyen Minh Hien2,*
1Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam;
2Research Group in Pharmaceutical and Biomedical Sciences, Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam
*Corresponding author: nguyenminhhien@tdtu.edu.vn
Received July 14th, 2025
Accepted August 18th, 2025
Summary
The microscopic study, high performance thin layer chromatography (HPTLC) profile, antioxidant, tyrosinase inhibitory properties, and anti-inflammatory properties of n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), water, and methanol (MeOH) extracts of Eurya nitida Korth. leaf were investigated for the first time. Besides total phenolic content (TPC) and total flavonoid content (TFC), the antioxidant determinations encompassed 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, total antioxidant capacity (TAC), ferric reducing antioxidant power (FRAP), and reducing power (RP). EtOAc extract exhibited the most potent radical scavenging capacities for DPPH (IC50 = 11.10 ± 0.18 µg/mL), ABTS (IC50 7.63 ± 0.04 µg/mL), RP (OD0.5 78.65 ± 0.63 µg/mL), FRAP (320.31 ± 14.34 mg AAE/g), TAC (123.2 ± 3.88 mg AAE/g), as well as significantly high TPC (292.41 ± 2.47 mg GAE/g) and TFC (76.30 ± 9.87 mg QE/g). Moreover, the EtOAc extract was found to possess the strongest anti-tyrosinase activity (37.96 ± 0.80% inhibition at 1000 µg/mL) among all tested samples. In addition, anti-inflammatory properties were also evaluated. Among all extracts, the EtOAc extract showed the highest inhibition of nitric oxide (NO) production in LPS-activated RAW 264.7 macrophages (IC50 25.62 ± 1.38 μg/mL), without showing cytotoxicity. In conclusion, Eurya nitida Korth. leaf presents a promising source of natural products for therapeutic applications in mitigating oxidative stress and anti-inflammation.
Keywords: Antioxidant; Tyrosinase inhibition; Anti-inflammation; HPTLC fingerprinting analysis; Microscopic characteristics; Eurya nitida Korth. leaf extracts.
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