Journal of Medicinal Materials, 2025, Vol. 30, No.3 (pp. 179 - 184)

OPTIMIZATION OF DAPHNORETIN EXTRACTION

FROM WIKSTROEMIA INDICA (L.) C.A.MEY TWIGS AND LEAVES

USING RESPONSE SURFACE METHODOLOGY

Luong Le Uyen Trang¹, Pham Van Tan¹, Nguyen Tra My², Vu Thi Diep²,

Nguyen Thi Thu², Nguyen Van Lieu^2,3, Nguyen Hoang Tuan⁴, Do Thi Ha^2,*

¹Hanoi Medical College, Vietnam;

²National Institute of Medicinal Materials (NIMM), Hanoi, 11022, Vietnam;

³Institute of Pharmaceutical Training, Vietnam Military Medical University, Vietnam;

⁴Faculty of Pharmacognosy and Traditional Medicine, Hanoi University of Pharmacy, Vietnam

*Corresponding author: hado.nimms@gmail.com

Summary

This study developed an optimized process for extracting and preparing a daphnoretin-rich extract from Wikstroemia indica (L.) C.A.Mey twigs and leaves using the One-Factor-At-A-Time (OFAT) method combined with Response Surface Methodology (RSM). Extraction was performed by simple soaking at room temperature. The optimal conditions were as follows: 1-2 mm material size, single extraction, 80% ethanol as the solvent, 26-hour extraction time, and a solvent-to-material ratio of 12:1. At a batch scale of 1 kg, the optimized process yielded an extract containing 2.481% daphnoretin with an extraction efficiency of 10.15%. This study highlights the feasibility of scaling up the process for industrial applications, offering a simple, efficient, and cost-effective method to produce high-quality daphnoretin-rich extracts.

Keywords: Wikstroemia indica; Daphnoretin; Extraction optimization; Response surface methodology; One factor at a time.

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