Journal of Medicinal Materials, 2025, Vol. 30, No. 5 (359 - 367)

SIMULTANEOUS DETERMINATION

OF CHLOROGENIC ACID AND ISOCHLOROGENIC ACID A

IN PHARBITIDIS SEMEN BY HPLC METHOD

Pham Thi Hien¹, Nguyen Thi Ha Ly¹, Nguyen Thi Kim Oanh^1,2, Nguyen Manh Khoa¹,

Nguyen Huy Van³, Tran Quang Luc³, Vu Huong Thuy³, Nguyen Thi Van Anh³,

Nguyen Thi Vinh Hue^3,4, Do Thi Ha^1,*

¹National Institute of Medicinal Materials (NIMM), Hanoi, 11022, Vietnam;

²Thai Binh University of Medicine and Pharmacy, Thai Binh, Vietnam;

³Traphaco Joint Stock Company, Hanoi, Vietnam;

⁴Faculty of Pharmacy, Dainam University, Ha Dong, Ha Noi 12119, Vietnam

*Corresponding author: hado.nimms@gmail.com

Summary

High-performance liquid chromatography (HPLC) was employed to quantify chlorogenic acid (CGA) and isochlorogenic acid A (ICGA) in Pharbitidis Semen, owing to its high sensitivity and suitability for quantitative analysis. An Agilent Eclipse Plus C₁₈ column (250 × 4.6 mm, 5 µm) and a DAD detector at 327 nm were employed for separation and detection. The mobile phase consisted of 0.1% formic acid in water and acetonitrile, with a flow rate of 1.0 mL/min and a 5 µL injection volume. The method demonstrated linearity over the ranges of 4.6 – 557.62 μg/mL for CGA and 4.3 – 557.62 μg/mL for ICGA. Validation parameters, including accuracy, system suitability, linearity, repeatability, precision, limit of detection (LOD), and limit of quantification (LOQ), met the AOAC requirements according to ICH guidelines. The findings are useful for quality control of Pharbitidis Semen in Vietnam.

Keywords: Pharbitidis Semen; HPLC-DAD; Acid chlorogenic; Isochlorogenic acid A.

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