Summary

Study on Callus Induction of Tea Tree (Melaleuca alternifolia) and Identification of

Terpinen-4-ol Content in Cultures

The leaves explants of in vitro cultured Melaleuca alternifolia plantlets were used as initial materials for cultures. The leaves were cut horizontally into two pieces and cultured on MS (1962) medium containing 2.4-D (4 mg/l), BA (0.5 mg/l), sucrose (20 g/l), vitamine B1 (5 mg/l) and glycine (10 mg/l) ) in dark at 26+2^oC for callus induction. After 40 days of culture, the rapidly growing white and soft calli were cultured on MS medium supplemented with 2.4-D (1 mg/l), sucrose (20 g/l), coconut water (15% v/v), glycine (10 mg/l) and vitamine B1 (5 mg/l) in dark, at ở 26+2 ^oC for cell masses induction and fast multiplication. After 25 days, cell masses were subcultured on liquid MS medium containing 2,4-D (1 mg/l), sucrose (20 g/l), coconut water (15% v/v), glycine (10 mg/l), vitamine B1 (5 mg/l) and yeast extract (150 mg/l) for callus fast multiplication and Terpinen-4-ol induction under low light condition (22.2 µmol/m²/s) at 26+2 ^oC, 14 h/day light cycle. The calli were formed under the conditions above containing Terpinen-4-ol reached to 0.0241 % after 15 days of liquid culture, could be used as materials for Terpinen-4-ol isolation.

Keywords: Callus, Melaleuca alternifolia, suspension cultures, terpinen-4-ol.

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