Summary

Study on Hyperglycemic Model in Bilaterally Ovariectomized Mice

The study was designed to perform hyperglycemic model in bilaterally ovariectomized mice (OVX mice). At two weeks after ovariectomy, hyperglycemia was induced in overnight-fasted OVX mice by intraperitoneal injections of streptozotocin (STZ) at dose of 50 mg/kg designed by two protocols: 3-time injections for every two days or 5-day consecutively injections. At 2^nd week or 4^th week after STZ administration, fasted plasma glucose levels were determined (initial plasma glucose). Mice were then subjected to oral glucose tolerance test and plasma glucose levels were measured again after 30 min and 120 min. The present study showed that bilateral ovariectomy induced a more severe impairment of glucose tolerance in diabetic mice at 2^nd week and 4^th week after STZ administration. Five-day consecutively intraperitoneal injections of STZ in OVX mice induced diabetic model which were more characteristic than three-time injections. Thus, this model might be useful for experimental study on drug treatment of glucose metabolism disorder in menopausal women.

Keywords: Bilateral ovariectomy (OVX), Streptozotocin (STZ), Hyperglycemic model