The Effect on the Ethanol-Induced Locomotor Activity and
Subchronic Toxicity of two Herbal Formulae in Mice
Nguyen Nhu Nguyet, Tran Thi Thu Giang, Nguyen Ngoc Khoi*
School of Pharmacy - University of Medicine and Pharmacy Ho Chi Minh City
*Corresponding author: nnkhoi@yahoo.com
(Received September 04th, 2013)
Summary
In order to evaluate the role of the combinations of Z. officinale, P. major extracts (2:1 for CT5) and these two extracts with C. sinenesis extract (1:1:1 for CT7) in mediating ethanol-induced locomotor activity, mice were pretreated for 7 days with CT5 and CT7 (0.6 g/kg) prior to one ethanol intraperitoneal injection (3.5 g/kg). After ethanol injection for 2 hours, locomotor activity of the mice was evaluated using open-field and rotarod apparatus. Ethanol injection showed significantly depressed locomotor activity compared with control group. Pretreatment with CT5 and CT7 enhanced locomotor activity in all ethanol[S1] -treated groups. The subchronic toxicity of CT5 and CT7 in mice also has been evaluated. In the subchronic study, mice were divided into three groups and were treated with normal diet (control), CT5 and CT7 (0.6 g/kg). After 60 days blood and tissue samples were taken for hematological, biochemical and histopathological determinations. Most of parameters did not alter significantly in all treated mice. Thus, the results indicate a low subchronic toxicity [S2] and confirm the ethanol-induced locomotor effects are enhanced by pretreated CT5 and CT7 formula in mice.
Keywords: Locomotion; Open field, Rota rod, Camellia sinensis; Zingiber officinale, Plantago major, Subchronic toxicity.
1. Introduction
In previous study, it was shown that a combination from Z. officinale, P. major extracts (2:1 for CT5) and these two extracts with C. sinenesis extract (1:1:1 for CT7) could be potential for prevent acute ethanol-induced toxicity [1, 2]. As a continuation of our study, the effect of these formulae on ethanol-induced locomotion was evaluated by open-field and rotarod apparatus. Furthermore, as part of safety evaluation of the formulae, the subchronic toxicity on mice of the above mentioned combinations was also studied.
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