Summary

Hairy-root Cultures of Artemisia annua L. in Semi-Solid and Liquid Cultures

Cytokinin does not stimulate hairy-roots proliferated but 2iP stimulates artemisinin accumulation. Auxin stimulates hairy-root proliferation but does not stimulate artemisinin accumulation. Supplemented with plant hormones on 14^th date has significantly stimulated hairy-root proliferation and artemisinin accumulation with the media of TH1 [B5 + GA3 (0.01mg/l) + ABA (0.1mg/l) + NAA (5mg/l)]. The improved MS media having sucrose (50g/l), N-nitrate (30mM), NH₄⁺/NO₃^- (1/5), KH₂PO₄ (1.5mM), GA3 (5mg/l), NAA (0.5mg/l) enhance artemisinin accumulation up to 1.468%.

Keywords: Hairy-root, accumulation, proliferation, semi-solid culture, liquid culture.

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