Acetovanillone Inhibits COX-2 Expression and Prostaglandin E2 Production in Raw264.7 Cells Via NF- kB and AP-1 Activity

The improper productions of NO and prostaglandins following the induction of cyclooxygenase-2 (COX-2) is involved in the pathogenesis of chronic inflammation. Belamcanda chinensis has been used as an oriental medicine for treatment of inflammation. Here, we isolated acetovanillone, irisflorentin, tectoridin, iristectorin A and iristectorigenin A from B. chinensis and demonstrated that acetovanillone inhibits the induction of COX-2 expression in RAW264.7 macrophages stimulated with lipopolysaccharide (LPS). Reporter gene assays and immunoblot analyses using subcellular fractionation revealed that transcription factor activities of nuclear factor-kB (NF-kB) and activator protein 1 (AP-1) were suppressed by acetovanillone. In addition, protein expression levels of p65, c-Jun and c-Fos were inhibited by acetovanillone treatment. Our findings indicate that acetovanillone may provide a developmental basis for an agent against inflammatory diseases.

Keywords: Belamcanda chinensis, Acetovanillone, COX-2, NF-kB, AP-1.

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