Research on Extraction, Isolation and Purification of Natural Compounds to Establish Reference Standards for Quality Control of Medicinal Materials and Herbal Medicines in Vietnam

The method for extraction, isolation and purification of ten natural compounds from medicinal materials for establishing the reference standards (RS) ((1) chlorogenic acid; (2) conessine; (3) holothurin B; (4) kaempferol; (5) linarine (budleoside); (6) malloapelta B; (7) myricetin; (8)nuciferin; (9) phyllanthin; and (10) silybin) were introduced. The objective substances were extracted from herbal materials by hot or cold extraction, then isolated by column chromatography (using different stationary phases i.e. silica gel, C_8, C₁₈ or Sephadex…). The purification consisted of using column chromatography or crystallization in solvents. The purified substances were essentially characterized by mass spectrometry and NMR. The chromatographic conditions for identification and quantification of purified substances were also experimentally established. The purity of almost target substances were greater than 95.0 %: chlorogenic acid 99.97%, conessine 97.80 %, holothurin B 96.17 %, kaempferol 95.21 %, linarin 95.10 %, malloapelta B 99.93 %, myricetin 99.69, nuciferine 100.11 %, phyllanthin 99.97 %, and silybin 100.15 %.

Keywords: Reference standards (RS), Establishment, Natural compounds, Extraction, Isolation, Purification crystallization.