Summary
Quality Evaluation of Rhei Rhizoma by HPLC
A simple high performance liquid chromatographic method was developed to evaluate the quality of Rhei Rhizoma based on chromatographic fingerprints that characterize 5 pharmacological compounds, namely, chrysophanol, emodin, emodin-glucoside, rhaponticin and sennoside A in 40 minutes. These compounds were identified by their characteristic UV profiles and mass spectroscopy data; their contents in Rhei Rhizoma samples were determined by HPLC. The chromatographic separation was performed on a C18 column by gradient elution with 0.05 M phosphoric acid in water and acetonitrile, the wavelength was set at 280 nm, the flow rate was set at 1 mL/min, gradient condition as followed: 0-5 min (20% B); 18 min (28% B); 25 min (42% B); 30 min (100% B) gave the satisfied separation. The methodological validation gave acceptable linearities (r >0.9995) and recoveries (ranging from 98.0-102.6%). The limits of detection (LOD) of these compounds ranged from 0.05 to 0.28 μg/mL. The developed method was applied to the quantitation of 31 samples of different Rhei Rhizoma species. The results showed that the contents of bioactive compounds were fluctuated among samples.
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