\n\tSummary
\n\tInhibitory Effect of Extracts from Chrysanthemum indicum in Nitrite Formation in LPS and HIV-1/ MULV-Induced Macrophage
\n\tThe macrophage represents one of the major target cell for human immunodeficiency virus (HIV) infection and is likely to play a major role in persistence and tissue dissemination of this virus. In the recent study we found that some extracts from Chrysanthemum indicum collecting from Vietnam exhibited the anti-HIV activity against two HIV-1 laboratory isolates (HIV-1IIIB and SF2). In this study we investigated the inhibitory effects of the active anti-HIV extracts F1,2,3 and 5 in NO production, aiming to understand the other biological effects, beneficial in treatment for people living with HIV/AIDS.
\n\tThe amount of NO produced by LPS or HIV-1/MuLV induced macrophages (5x105 cells/ml) was measured in terms of nitrite formation . Cells were plated in in 24 well culture plates in the presence of LPS or HIV-1/MuLV and allow to adhere for 24 hr. The amount of NO2⁻ measured by Griess reagent is correlated to the NO production by macrophage. Our extracts from Chrysanthemum indicum flowers significantly reduced the formation of nitrite on LPS or HIV-1/ MuLV-induced cells in a concentration-dependent manner. All tested extracts F1, F2, F3, F5 at 25 and 150 μM, was found to inhibite the NO formation significantly (P<0.01). F3 was found to be the most active in both cases of NO formation inhibition,which was also the extract showing the most anti-HIV effects.
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