Summary

Isolation of Ginsenoside-Rh₁ in Higher Yield from Processed Vietnamese Ginseng

The underground part of Panax vietnamensis has been used in raw forms for treating various diseases and for enhancing physical strength. In our previous studies, remarkable changes in saponin compositions were detected by comparative TLC and HPLC between fresh Vietnamese ginseng and that of the steamed one. This study investigated the change in saponin composition of processed Vietnamese ginseng by various isolation and analysis steps. Two among three isolated dammarane-type triterpenoid saponins were elucidated as 20(S) ginsenoside-Rh₁ and 20(R) ginsenoside-Rh₁ by using spectroscopic analyses including IR, MS and  NMR. Ginsenoside-Rh₁ was reported to be isolated in Vietnamese ginseng at very low yield (c.a. 0.02 %) [5, 6] . In our study, after steaming processed, ginsenoside Rh₁ were remarkably produced (yield 0.5 %) and for the first time, two chiral molecules 20(S) and 20(R) ginsenoside Rh₁ were isolated separately from Panax vietnamensis.

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