Journal of Medicinal Materials, 2024, Vol. 29, No. 2 (pp. 98 - 106)
OPTIMIZATION OF PRESSURIZED SOLVENT EXTRACTION
USING RESPONSE SURFACE METHODOLOGY AND HPLC ANALYSIS
OF VITEXIN FROM PASSIFLORA FOETIDA L.
Nguyen Thi Kim Anh1, Do Thi Thuy Linh1, Le Xuan Duy1, Nguyen Phu Quang1,
Phan Thanh Thuy1, Bui Thi Thuy Luyen2,٭
1National Institute of Medicinal Material (NIMM), Hanoi, Vietnam;
2Department of Pharmaceutical Chemistry Technology and Extraction, Faculty of Pharmaceutical Chemistry and Technology, Hanoi University of Pharmacy, Hanoi, Vietnam
*Corresponding author: luyenbtt@hup.edu.vn
(Received November 15th, 2023)
Summary
Optimization of Pressurized Solvent Extraction Using Response Surface Methodology and HPLC Analysis of Vitexin from Passiflora Foetida L.
The study aims to optimize the automated sample preparation step using accelerated solvent extraction (ASE) and response surface methodology (RSM) and integrate it with a simple, quick, precise, and accurate high performance liquid chromatography (HPLC) technique for vitexin measurement in P. foetida. The RSM results demonstrated that the optimal conditions for vitexin extraction were an extraction temperature of 150.59°C, an extraction time of 19.16 min, and a solvent volume of 140.91 mL; The actual values at the optimal conditions according to the model showed the maximum value of the objective function of vitexin yield was 11.18 ± 0.15 (mg/g). The validated HPLC method was as follows: A mixture of acetonitrile-0.1% formic acid solution with the elution gradients were: 0 - 40 min, 15% acetonitrile; 40 - 45 min, 100% acetonitrile. Other running conditions included the detection wavelength (342 nm), the flow rate (1 mL/min), the injection volume (20 µL), and the column temperature (40°C).
Keywords: Passiflora foetida L., Vitexin, Accelerated solvent extraction (ASE), High performance liquid chromatography (HPLC), Response surface methodology (RSM).
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