Summary

In Vitro Propagation of Polyscias fruticosa (L.) Harms by

Direct Regeneration from Shoot Tips

Shoot tips of 1.0 to 1.5 cm long taken from field growing Polyscias fruticosa (L.) Harms (Araliaceae) were sterilized and cultured in Murashige and Skoog (MS) medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IBA. After 8 weeks, these shoots began to swell at the internodes and gave rise to 2-3 new shoots after 4 more weeks. Subculture of the newly formed shoots in MS media containing different concentrations and combinations of BAP, IBA and NAA showed that 2.0 mg/l BAP + 0.5 mg/l IBA was the best combination for shoot proliferation. In this medium, an average of 8.87 ± 1.60 shoots was obtained from an initial shoot in about 10 weeks time. All shoots regenerated directly from axillary buds without visible callus formation. 100 % rooting of the shoots were induced in MS medium supplemented with 1.0 mg/l IBA + 0.5 mg/l BAP in less than 20 days. The plantlets were successfully transferred to PE bags under mist.

Keywords: Polyscias fruticosa,In vitroPropagation, Direct Regeneration, Shoot Tips. 

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