Development of a Method for Quantitative Analysis of Z-Ligustilid
in the Roots of Angelica acutiloba by High Performance Liquid Chromatography
The compound Z-ligustilid is one of major compounds of Danggui (Angelica spp) in the oriental medicine. In this report, we established a method using HPLC for quantitative analysis of this constituent from the root of Angelica acutiloba (Sieb. Et Zucc.) Kitagawa, known as “Duong qui di thuc” in Vietnam. Z-ligustilid was extracted from A. acutiloba using soxhlet extraction method. The analysis of this compound was carried out using a C(18) column (S-5 μm, 4.6 mm I.D. × 250 mm) and gradient elution with acetonitrile and water containing 0.1% phosphoric acid. The analytical method was validated for linearity, precision and accuracy. Calibration curve for the standard compound showed good linear regression (r2 > 0.998). The relative standard deviations (RSD %) of the retention time (tR), peak square, precision, and accuracy were 0.02 %, 0.05 %, 1.47 % and 1.87 %, respectively. The recovery of this method was 98.93%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.6 and 1.98 μg/mL, respectively. These results have indicated that the developed method was efficient for the quality evaluation of Japanese Danggui. The contents of Z-ligustilid in samples Duong qui di thuc collected in Vietnam were also determined.
Keywords: Angelica acutiloba, Z-Ligustilid, HPLC, Quantitative analysis.
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