Journal of Medicinal Materials, 2024, Vol. 29, No. 2 (pp. 106 - 112)

A QUANTITATIVE METHOD FOR DETERMINING CALYCOSIN-7-O-β-D-GLUCOSIDE AND FREE ASTRAGALOSIDE IV IN RAW

AND HONEY-PROCESSED ASTRAGALI RADIX BY HPLC-DAD

Nguyen Thi Ha Ly, Nguyen Thi Hang, Pham Thi Hien, Nguyen Thi Thu, Vu Thi Diep,

Tran Thi Huyen Trang, Le Thanh Nghi, Do Thi Ha*

 National Institute of Medicinal Materials (NIMM), Hanoi, Vietnam

*Corresponding author: hado.nimms@gmail.com

(Received January 03^st, 2024)

Summary

A Quantitative Method for Determining Calycosin-7-O-β-D-Glucoside and Free Astragaloside IV in Raw

and Honey-Processed Astragali radix by HPLC-DAD

An HPLC-DAD method was developed to quantify calycosin-7-O-β-D-glucoside and free astragaloside IV in raw and honey-processed Astragali Radix samples. The HPLC analysis was performed on an Agilent C₁₈ column (4.6 × 250 mm, 5 µm particle size), with a mobile phase of acetonitrile-0.2% formic acid, the flow rate of 1.0 mL/min, and detection wavelengths of 260 nm for calycosin-7-O-β-D-glucoside and 203 nm for astragaloside IV. All calibration curves displayed a good linear relationship (R²=0.999). The method was fully validated concerning the specificity, linearity, accuracy, and recovery according to the ICH guideline. The raw Astragali Radix samples had varying levels of calycosin-7-O-β-D-glucoside and free astragaloside IV, ranging from 0.016 - 0.032 % and 0.016-0.066 %, respectively. In the honey-processed Astragali Radix samples, the contents of free astragaloside IV and calycosin-7-O-β-D-glucoside were 0.018 - 0.052 % and 0.010 - 0.022 %, respectively.

Keywords: Astragali Radix, HPLC-DAD, Calycosin-7-O-β-D-glucoside, Free astragaloside IV.

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