Journal of Medicinal Materials, 2019, Vol. 24, No. 5 (pp.276 - 280)
A VALIDATED HPLC-PDA METHOD FOR SIMULTANEOUS QUANTITATION OF BERGENIN AND ASTILBIN IN THE UNDERGROUND PARTS OF ASTILBE RIVULARIS
Dinh Thi Quynh Anh1, Nguyen Quoc Tuan1,*, Nguyen Duc Hung1, Ha Thanh Hoa1,
Dao Viet Hung1, Ngo Xuan Thinh1, Nguyen Thi Minh Diep1, Pham Ngoc Khanh2, Pham Quoc Tuan1,*
1Centre for Drug Research and Technology Transfer -
Phu Tho College of Medicine and Pharmacy, Vietnam;
2Sa Pa Medicinal Plant Research Station - National Institute of Medicinal Materials, Vietnam
*Corresponding author: email@example.com or firstname.lastname@example.org
(Received September, 09th, 2019)
A Validated HPLC-PDA Method for Simultaneous Quantitation of Bergenin and Astilbin in the Underground Parts of Astilbe rivularis
For the first time, the content of bergenin and astilbin in the underground parts of Astilbe rivularis were determined using an HPLC method. The chromatographic condition included analytical column YMC C18 (150 x 4.6 mm; 5 μm); the mobile phase was methanol and water. The chromatographic program was followed as 0-60 min, 15-100% MeOH; flow rate was 0.8 mL/min; UV detection was recorded at 216 nm for both bergenin and astilbin. The calibration curves displayed excellent linearity (R2 = 0.9999 for bergenin and R2 = 0.9996 for astilbin), and high sensitivity (LOD and LOQ of bergenin were 2.50 and 8.25 µg/mL, respectively; LOD and LOQ of astilbin were 3.50 and 11.55 µg/mL, respectively). The chromatographic system was achieved in good repeatability with relative standard deviation (RSD) of 0.30%. The recovery averages of bergenin and astilbin were 96.84% and 97.37%, respectively.
Keywords: Bergenin, Astilbin, Astilbe rivularis, HPLC, Quantitation.
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