Journal of Medicinal Materials, 2019, Vol. 24, No. 5 (pp.276 - 280)

A VALIDATED HPLC-PDA METHOD FOR SIMULTANEOUS QUANTITATION OF BERGENIN AND ASTILBIN IN THE UNDERGROUND PARTS OF ASTILBE RIVULARIS

            Dinh Thi Quynh Anh¹, Nguyen Quoc Tuan^1,*, Nguyen Duc Hung¹, Ha Thanh Hoa¹,

Dao Viet Hung¹, Ngo Xuan Thinh¹, Nguyen Thi Minh Diep¹, Pham Ngoc Khanh², Pham Quoc Tuan^1,*

¹Centre for Drug Research and Technology Transfer -

Phu Tho College of Medicine and Pharmacy, Vietnam;

²Sa Pa Medicinal Plant Research Station - National Institute of Medicinal Materials, Vietnam

*Corresponding author: phamquoctuan@duocphutho.edu.vn or quoctuan301281@gmail.com

(Received September, 09^th, 2019)

Summary

A Validated HPLC-PDA Method for Simultaneous Quantitation of Bergenin and Astilbin in the Underground Parts of Astilbe rivularis

For the first time, the content of bergenin and astilbin in the underground parts of Astilbe rivularis were determined using an HPLC method. The chromatographic condition included analytical column YMC C₁₈ (150 x 4.6 mm; 5 μm); the mobile phase was methanol and water. The chromatographic program was followed as 0-60 min, 15-100% MeOH; flow rate was 0.8 mL/min; UV detection was recorded at 216 nm for both bergenin and astilbin. The calibration curves displayed excellent linearity (R² = 0.9999 for bergenin and R² = 0.9996 for astilbin), and high sensitivity (LOD and LOQ of bergenin were 2.50 and 8.25 µg/mL, respectively; LOD and LOQ of astilbin were 3.50 and 11.55 µg/mL, respectively). The chromatographic system was achieved in good repeatability with relative standard deviation (RSD) of 0.30%. The recovery averages of bergenin and astilbin were 96.84% and 97.37%, respectively.

Keywords: Bergenin, Astilbin, Astilbe rivularis, HPLC, Quantitation.

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