Journal of Medicinal Materials, 2022, Vol. 27, No. 1 (pp. 19 - 24)
ACETYLCHOLINESTERASE INHIBITORY ACTIVITY OF
FATTY ACIDS ISOLATED FROM BROWN SEAWEED
SARGASSUM MCCLUREI SETCHELL
Ha Thi Ngoc Tram1, Do Nguyen Phuong Nam1, Nguyen Thi Ngoc Dung1,*,
Nguyen The Han2,*, Vo Thi Bach Hue3, Phan Van Ho Nam1
1Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam;
2Faculty of Food Technology, Nha Trang University, Nha Trang City, Vietnam;
3Faculty of Pharmacy, Lac Hong University, Dong Nai, Vietnam
*Corresponding author: firstname.lastname@example.org or email@example.com
(Received January 25th, 2022)
Acetylcholinesterase Inhibitory Activity of Fatty Acids Isolated from Brown Seaweed Sargassum mcclurei Setchell
The 80% methanol extract of Sargassum mcclurei (brown seaweed) showed a significant acetylcholinesterase (AChE) inhibitory activity. The total extract at a concentration of 25 µg/ml inhibited 47 ± 11% of AChE activity. By using organic solvents with increasing polarity, four fractions (n-hexane, dichloromethane, n-butanol and water) were obtained. n-Hexane fraction showing 42% inhibition at 5000 µg/ml was then fractionated by column chromatography in n-hexane-ethyl acetate to obtain two pure compounds, identified as palmitic acid and oleic acid. Especially palmitic acid at a concentration of 300 µg/ml could inhibit 81 ± 3% AChE activity (IC50 = 100 ± 7 µg/ml).
Keywords: Sargassum mcclurei, Acetylcholinesterase inhibitory activity, Palmitic acid, Oleic acid.
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