Journal of Medicinal Materials, 2021, Vol. 26, No. 5 (pp. 294 - 300)
ANALYSIS OF PESTICIDE RESIDUES IN BETEL’S EXTRACT
(PIPER BETLE L. PIPERACEAE) BY GC - MS/MS METHOD
Pham Ben Chi1,*, Vo Thi Bach Hue2, Nguyen Dinh Nga3, Nguyen Thi Ngoc Van4
1Faculty of Medical Laboratory Technique, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam.
2Faculty of Pharmacy, Lac Hong University, Bien Hoa City, Dong Nai Province, Vietnam;
3Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam;
4Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, Can Tho City, Vietnam
*Corresponding author: pbchi@ntt.edu.vn
(Received June 05th, 2021)
Summary
Analysis of Pesticide Residues in Betel’s Extract (Piper betle L. Piperaceae) by GC - MS/MS Method
This research studied the sample’s process and qualification of pesticide residues in betel’s extract by GC - MS/MS. The pesticide residues were extracted by modified QuEChERS: using 1% of acid acetic in acetonitrile (ACN) as extracted solvent; separating the ACN layer and water layer by using mix of MgSO4 4 g and NaCl 1 g; cleaning - up by dSPE (MgSO4 1 g, PSA 0.4 g, C18 0.4 g) and liquid - liquid partition technique with n - hexane and NaCl 20% (w/v). Basing on the guideline of AOAC 2016 and EC 2018, the quantified method showed good results of system suitability, specificity, linearity within the concentration range of 0 - 200 ppb (R2 >0.99), repeatability (RSD < 20%), and recovery (63.84 - 106.06%). Therefore, the analytical method can be applied for quantifying the pesticide residues in betel’s extract which contributes to quality control and standardization of the betel’s extract.
Keywords: Betel’s extract, Pesticide residues, QuEChERS, GC - MS/MS.
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