Ấn phẩm

Antioxidant and Anti-Inflammatory Potential from Ochna integerrima Stem (Tạp chí Dược liệu 2023, tập 28, số 3)

Journal of Medicinal Materials, 2023, Vol. 28, No. 3 (pp. 185 - 191)

 

ANTIOXIDANT AND ANTI-INFLAMMATORY POTENTIAL

FROM OCHNA INTEGERRIMA STEM

Nguyen Thi Huong Nguyen1, Phan Thi Linh2, Le Pham Thuy An1, Nguyen Phuc Khanh1,

Nguyen Hung An2, Ton Nu Lien Huong2, Nguyen Bao Anh1, Le Thi Kim Chi3,

Nguyen Minh Hien1,*

1Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam;

2College of Natural Sciences, Can Tho University, Vietnam;

3Department for Facility Management, Ton Duc Thang University, Ho Chi Minh City, Vietnam

*Corresponding author: nguyenminhhien@tdtu.edu.vn

(Received May 09th, 2023)

Summary

Antioxidant and Anti-Inflammatory Potential from Ochna integerrima Stem

The present work aims to study the antioxidant activity, the anti-inflammatory effects of n-hexane, ethyl acetate (EtOAc), n-BuOH, aqueous, and methanol (MeOH) extracts, and chemical components from n-hexane extract. The antioxidant potential was determined through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. The total phenolic (TPC) and flavonoid contents (TFC) were measured using the Folin-Ciocalteu (FC) and aluminum chloride assays. The anti-inflammatory activity of the extracts was evaluated as the inhibition of bovine serum albumin (BSA) denaturation. All extracts showed significant antioxidant activity, except for the n-hexane extract. Among the extracts, the aqueous extract had the most potent antioxidant activity in DPPH and ABTS assays with IC50 values of 9.57 ± 0.21 µg/mL and 3.6 ± 0.15 µg/mL; and with high TPC and TFC values (263.34 ± 6.83 mg GAE/g) and (5.19 ± 0.16 mg QE/g), respectively. Similarly, it also showed the greatest FRAP value of 304.30 ± 25.15 mg AAE/g.  For the anti-inflammatory activity, the inhibition of BSA denaturation was ranked as n-hexane extract (57.11%) > aqueous extract (49.80%) > n-BuOH extract (31.94%) > MeOH extract (27.96%) > EtOAc extract (15.63%) at the concentration of 125 mg/mL. The two steroids stigmasterol-3-O-β-ᴅ-glucopyranoside (1) and stigmasterol (2) were isolated to prove the anti-inflammatory activity of n-hexane extract.  To sum up, the extracts of O. integerrima stem may have therapeutic potential against oxidative stress-related and inflammatory disorders. For the first time, O. integerrima was evaluated for its anti-inflammatory activity using heated-induced BSA denaturation.

Keywords: Antioxidant, Anti-inflammatory, Extracts, O. integerrima stem.

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