Journal of Medicinal Materials, 2019, Vol. 24, No. 5 (pp. 308 - 314)

DETERMINATION OF AFLATOXIN IN HERBAL MEDICINES

BY LIQUID CHROMATOGRAPHY COUPLED

WITH TANDEM MASS SPECTROMETRY (LC-MS/MS)

Nguyen Dinh Quan^1,*, Nguyen Thi Phuong¹,

Ha Anh Tuan², Nguyen Minh Khoi¹, Phuong Thien Thuong³

¹National Institute of Medicinal Materials, Hanoi, Vietnam;

²College of Pharmacy, Vietnam National University, Hanoi;

³Vietnam - Korea Institute of Science and Technology, Vietnam

*Corresponding author: quandn.dkh@gmail.com

(Received September, 15^th, 2019)

Summary

Determination of Aflatoxin in Herbal Medicines by Liquid Chromatography Coupled

with Tandem Mass Spectrometry (LC-MS/MS)

In this study, a method using LC-MS/MS was established for the quantitative analysis of aflatoxin in medicinal plants. The aflatoxins were extracted, purified by immunoaffinity column chromatography (SPE-IM) and analyzed by high-performance liquid chromatography coupled with tandem quadrupole mass spectrometry with electrospray ionization (LC-MS/MS). The aflatoxins can be separated within 6.5 min using a Shimpack C₁₈ (100 × 2.1 mm, 1.9 µm). Chromatographic analyses were carried out using binary gradient elution with an isocratic solvent system of 60: 40, v/v acetonitrile with 10 mM aqueous ammonium acetate. A good linear relationship was found for AFB1, AFB2, AFG1, and AFG2 at 0.1 - 10 ppb (r²>0.99). The analyte accuracy under three different spiking levels was 90.13% to 101.47%, with low percentage of relative standard deviations in each case. We found that AFs were in Semen Coicis purchased in Ninh Hiep market. The analytical method developed is simple, precise, accurate, economical and can be effectively used to determine the aflatoxins in herbal medicines and therefore to control the quality of products.

Keywords: Aflatoxin, Herbal medicines, SPE-IM, LC-MS/MS.

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