Journal of Medicinal Materials, 2024, Vol. 29, No. 3 (pp. 158 - 163)
DETERMINATION OF CHLORPHENIRAMINE ADULTERATED
IN HERBAL PRODUCTS USING HIGH PERFORMANCE
THIN LAYER CHROMATOGRAPHY
Nguyen Thi Thuy Linh1, Dao Thi Cam Minh2, Le Thuy Linh1, Dang Thi Ngoc Lan1, Vu Ngan Binh1, Pham Thi Thanh Ha1,*
1Hanoi University of Pharmacy, Vietnam;
2 Hue University of Pharmacy and Medicine, Hue University, Vietnam
*Corresponding author: thanhha.pham@hup.edu.vn
(Received May 16th, 2024)
Summary
Determination of Chlorpheniramine Adulterated in Herbal Products
Using High Performance Thin Layer Chromatography
A high-performance thin layer chromatographic (HPTLC) method was established for the identification, qualitative, and quantitative analysis of chlorpheniramine adulterated in herbal products. The samples were extracted with methanol, followed by TLC separation with cyclohexane-acetone-ammonia as mobile phase and UV scanning detection at 254 nm. The method was validated according to AOAC 2016 guidelines, which showed high specificity, good precision, and accuracy. The validated method was applied to 60 herbal products collected from the market via online purchase, at private pharmacies, or from traditional healthcare practitioners. Chlorpheniramine was detected in 18/60 products.
Keywords: Chlorpheniramine, Adulteration, Herbal products, High performance thin layer chromatography.
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