Journal of Medicinal Materials, 2024, Vol. 29, No. 6 (pp. 354 - 361)
DEVELOPMENT AND VALIDATION OF QUANTITATIVE ANALYSIS
OF L-DOPA FROM MUCUNA PRURIENS
Le Thi Loan1, Dang Minh Tu1, Dang Viet Hau1, Nguyen Thi Thu Trang1, Luong Thi Lan1,
Ha Van Oanh2, Nguyen Van Tai1,*
1National Institute of Medicinal Materials (NIMM), Hanoi, Vietnam;
2Hanoi University of Pharmacy, Vietnam
*Corresponding author: nguyenvantai1111@gmail.com
(Received February 28th, 2024)
Summary
Development and Validation of Quantitative Analysis of L-Dopa from Mucuna pruriens
Mucuna pruriens is an important medicinal plant used for the treatment of Parkinson’s disease and many others in ancient traditional Indian medicine. This study reports a simple and rapid high performance liquid chromatography (HPLC) method for the quantification of L-dopa in Mucuna pruriens seeds. Methanol/water/hydrochloric acid (70:30:0.1) was used as the extraction solvent. The chromatographic condition was performed on the Agilent Zorbax Eclipse plus C18 column (250 x 4.6 mm, 5 µm), the mobile phase was 0.1% acetic acid/methanol (99:1), flow rate 0.8 mL/min, and the ultraviolet (UV) detector was set at 280 nm. The method has been validated, showing that it is selective, specific, accurate, and suitable for the quantification of L-dopa in Mucuna seeds. The recovery was 99.89 - 101.49%, and relative standard deviation (RSD) values of the repeatability and intermediate precision were less than 2%. The limit of quantification of L-dopa was 0.12 µg/mL, corresponding to 0.07 mg/g in samples. This method was used to determine the L-dopa content in 5 samples of Mucuna pruriens seeds and showed the L-dopa content ranging from 5.20 ± 0.02% to 5.92 ± 0.10% by weight of dried medicinal herbs.
Keywords: Mucuna pruriens, L-dopa, Levodopa, HPLC.
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