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HPLC Analysis of Lobetyolin in Radix Codonopsis javanicae (Journal of Medicinal Materials, 2021, Vol. 26, No. 5)

Journal of Medicinal Materials, 2021, Vol. 26, No. 5 (pp. 282 - 288)



Nguyen Truong Huy1, Nguyen Thi Minh Trang1, Huynh Tran Quoc Dung2,

Ngo Thi My Duyen1, Tran Mong Kha1, Nguyen Minh Duc1,*

1Falculty of Pharmacy, Ton Duc Thang University, Vietnam;

2Traditional Medicine Hospital, Ho Chi Minh City,Vietnam

*Corresponding author: nguyenminhduc@tdtu.edu.vn

(Received April 15th, 2021)


HPLC Analysis of Lobetyolin in Radix Codonopsis javanicae


Codonopsis javanica roots have long been used as a popular remedy in Vietnam traditional medicine. Despite its popularity, the current monograph of Radix Codonopsis javanicae in Vietnamese Pharmacopoeia V (2017) only describes TLC qualitative analysis of lobetyolin, but no assay is mentioned. This study, therefore, aimed to develop and validate a reliable and effective quantitative HPLC-UV method for assaying lobetyolin, the officially accepted biomarker of Radix Codonopsis javanicae. The chromatographic separation was performed on Luna C18 column using gradient elution of water and acetonitrile, while the method was validated in terms of specificity, linearity (r2 > 0.999), precision [relative standard deviation (RSD) < 1.19%], and accuracy (98 - 102%). This validated method was subsequently applied to quantify the lobetyolin content in 2- and 3- year roots of Codonopsis javanica collected in Lam Dong province. The result showed that the HPLC-UV method was proven to be effective for the assay and the average content of lobetyolin in the 2- and 3-year Codonopsis roots were 0.066% and 0.206%, respectively. Thus, the established HPLC-UV method can be useful for assaying lobetyolin in the crude drug.

Keywords: HPLC-UV method, Codonopsis javanica, Lobetyolin.

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