Journal of Medicinal Materials, 2023, Vol. 28, No. 6 (pp. 366 - 371)
IN VITRO ANTI-INFLAMMATORY ACTIVITIES OF FISSISPALLIN B ISOLATED FROM FISSISTIGMA PALLENS
Trieu Ha Phuong1,3, Le Nguyen Hai Giang3, Nguyen Thi Cuc1, Nguyen Thi Nga1,3,
Do Thi Phuong1, Le Hoang Ngan Ha1, Tran Le Nam Khanh1, Do Thi Thao1,2,*
1Institute of Biotechnology, Vietnam Academy of Science and Technology;
2Graduate University of Science and Technology, Vietnam Academy of Science and Technology;
3University of Science and Technology of Ha Noi, Vietnam Academy of Science and Technology
*Corresponding author: thaodo@ibt.ac.vn
(Received October 16th, 2023)
Summary
In vitro Anti-Inflammatory Activities of Fissispallin B Isolated from Fissistigma pallens
Inflammation refers to the process from the body against stimulation of infections, injuries, toxins, etc. Acute inflammation could become chronic with a category of other serious symptoms and diseases. Being a rich source of potential bioactivities, plants and their chemical components present a promising reservoir for developing new, safer anti-inflammatory drugs. Fissistigma pallens (Finet & Gagnep.) Merr. (Annonaceae), growing in the Northern regions of Vietnam has been used in traditional medicine to treat several diseases. In this study, the anti-inflammatory activity of fissispallin B (FPB) isolated from F. pallens was evaluated. Its nitric oxide (NO), tumor necrosis factor (TNFa), and interleukin 6 (IL-6) inhibitory activity and IL-10 stimulatory activity using LPS-induced RAW264.7 macrophage inflammatory model. The compound illustrated its promising NO inhibitory effects with IC50 values of 2.24 ± 0.17 µM. Besides, fissispallin B significantly expressed the inhibiting capacity on pro-inflammatory cytokines (IL-6, TNF-a) while it had not much effect on stimulation of anti-inflammatory IL-10 cytokine. Treatment of the fissispallin B at 2.5 μM and 5 μM in RAW 264.7 cells remarkably decreased NF-kB protein expression to 36.1% and 51.2% compared to that of the control, respectively (P<0.05) by using Western-blot analysis. On the other hand, the high percentage of cell viability indicated this compound had a weak cytotoxic effect in this experiment. In conclusion, fissispallin B showed potential in vitro anti-inflammatory bioactivity.
Keywords: Fissistigma pallens, RAW264.7, Anti-inflammation, Nitric oxide, Fissispallin B (FPB).
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