Journal of Medicinal Materials, 2022, Vol. 27, No. 4 (pp. 213 - 218)
Investigation OF CHEMICAL CONSTITUENTS OF
CARDIOSPERMUM HALICACABUM L. AND THEIR BIOLOGICAL ACTIVITIES
Nguyen Thi Thuy Linh1,2, Ba Thi Cham1,2, Nguyen Thi Hoang Anh1,2, Nguyen Phi Hung1,3, Do Thi Thao1,4, Nguyen Thi Loan5, Le Thi Hong Nhung5, Domenico V Delfino6, Trinh Thi Thuy1,2,*
1Graduate University of Science and Technology, Vietnam Academy Science and Technology;
2Institute of Chemistry, Vietnam Academy Science and Technology;
3Institute of Natural Products Chemistry, Vietnam Academy Science and Technology;
4Institute of Biotechnology, Vietnam Academy Science and Technology;
5Hanoi University of Industry, Vietnam;
6Department of Medicine and Surgery, University of Perugia, Perugia, Italy
*Corresponding authors: thuy@ich.vast.vn
(Received May 09th, 2022)
Summary
Investigation of Chemical Constituents of
Cardiospermum halicacabum L. and their Biological Activities
Four compounds were isolated from n-hexane and n-butanol extracts of Cardiospermum halicacabum growing in Vietnam by combined column chromatographic methods. Based on 1D-, 2D-NMR, ESI MS analyses, and comparisons with those published in work of literatures, the structures of these compounds were established as 3β-hydroxyglutin-5-ene (1), zerumbone (2), uridine (3), and daucosterol (4). It is intriguing to point out that, three out of them (1-3) were detected for the first time from the family Sapindaceae. Additionally, the cytotoxic activity of compound 3 was assessed against five human cancer cell lines (A549, T24, Huh-7, 8505, SNU-1) using the SRB method. Additionally, 3 was also evaluated for immunomodulatory capacity through stimulation of IL-2 expression in macrophages (RAW264.7). Compound 3 significantly stimulated IL-2 expression in RAW264.7 cells at a concentration of 100 µg/mL (P<0.05) while no affection was observed at all lower tested concentrations.
Keywords: Cardiospermum halicacabum L., Uridine, Immunomodulatory effect, Cytotoxic activity.
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