Journal of Medicinal Materials, 2022, Vol. 27, No. 2 (pp. 123 - 128)
METHOD DEVELOPMENT FOR QUANTIFICATION
OF PAEDEROSIDIC ACID FROM PAEDERIA SCANDENS
BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Do Thi Thuy Linh, Hoang Thanh Duong, Do Quang Thai, Pham Anh Minh, Tran Anh Quang, Nguyen Tuan Hiep, Tran Thanh Ha*
National Institute of Medicinal Materials
*Corresponding author: email@example.com
(Received February 14th, 2022)
Method Development for Quantification of Paederosidic Acid from Paederia scandens
by High Performance Liquid Chromatography
A simple and sensitive high performance liquid chromatography (HPLC-DAD) method for quantification of paederosidic acid from Paederia scandens aerial part was developed and validated. The chromatographic separations were obtained by a Shimadzu C18 column (250×4.6mm, 5µm) using isocratic mode with 0.1% phosphoric and acetonitrile (85:15, v/v). Detection was set at a wavelength of 234 nm. The validation results displayed good accuracy, repeatability, linearity, and selectivity. The contents of paederosidic acid in Paederia sc acid andens samples were 0.0075 -0.2339%. The obtained results provide useful information for the quality testing of the Paederia scandens in Vietnam.
Keywords: Paederia scandens (Lour.) Merr., Paederosidic acid, HPLC.
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