Journal of Medicinal Materials, 2018, Vol. 23, No. 3 (pp. 157 - 166)
OPTIMIZATION OF EXTRACTION CONDITIONS FOR ACHIEVING HIGH CONTENT AND ANTIOXIDANT ACTIVITIES OF THE TOTAL PHENOLIC COMPOUNDS OF PHU QUOC SIM FRUIT (RHODOMYRTUS TOMENTOSA (AITON) HASAK.)
Le Phuong Uyen1, Ngo Dai Hung2, Vo Thanh Sang1,*
1NTT Institute of Hi-Technology, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam;
2Faculty of Natural Sciences, Thu Dau Mot University, Binh Duong Province, Vietnam
*Corresponding author: email@example.com
(Received November, 13th, 2017)
Optimization of Extraction Conditions for Achieving High Content and Antioxidant Activities of
the Total Phenolic Compounds from Phu Quoc Sim Fruit (Rhodomyrtus tomentosa (Aiton) Hasak.)
Rhodomyrtus tomentosa is an evergreen shrub that originates in Southeast Asia and grows in abundance with rose-pink flowers and dark-purple edible bell-shaped fruits. In this study, optimal extraction conditions for achieving high content and antioxidant activities of the total phenolic compound (TPC) from Phu Quoc sim fruit was investigated. It was found that the total phenolic content was achieved upon 50.9 ± 1 mg gallic acid equivalent (GAE)/g dry weight extract (DWE) and the DPPH scavenging activity was 60.8 ± 2% at concentration of 1000 µg/ml. The optimal extraction conditions were 100% methanol, 3 h of extraction time at temperature of 40°C, solvent-to-solid ratio of 2/1 (v/w). Furthermore, the fractionation of ethyl acetate (EtOAc) was revealed to possess the highest TPC and antioxidant activities among n-hexane, dichloromethane, butanol, and water residue. TPC of EtOAc was 326.4 ± 1 mg GAE/g DWE, meanwhile its antioxidant activities were found due to scavenging DPPH (IC50 = 368.1 µg/ml) and ABTS (IC50 = 134.5 µg/ml) and protecting DNA from damage induced by H2O2. Accordingly, Phu Quoc sim fruit was suggested as potential source of bioactive polyphenols with high antioxidant activities.
Keywords: Sim, Rhodomyrtus tomentosa, Phenolic, DPPH, Antioxidant.
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