Journal of Medicinal Materials, 2023, Vol. 28, No. 2 (pp. 99 - 103)

PHYTOCHEMICAL CHARACTERIZATION AND BIOACTIVITY EVALUATION OF ARDISIA SYLVESTRIS LEAVES FROM LAM BINH MOUNTAINOUS DISTRICT, TUYEN QUANG PROVINCE

Nguyen Trung Thanh¹, Dang Hong Duyen¹, Pham Thi Thu Trang¹, Nguyen Thi Thu Minh¹,

Nguyen Thi Hai², Hua Van Luong³, Chu Quynh Mai ⁴, Doan Thi Phuong Ly⁴, Nguyen Thi Minh Hue⁴, Tran Thi Thanh Van⁴, Ngo Thi Thuy Ngan⁵, Nguyen Thi Thu Thuy⁶, Nguyen Tien Dat^1, *, Do Hoang Giang^1, *

¹Center for Research and Technology Transfer, Vietnam Academy of Science and Technology;

²Ministry of Education and Training, Vietnam;

³Lam Binh Forest Protection Office, Tuyen Quang Province; ⁴Tan Trao University, Vietnam;

⁵Department of Fundamental Science, Thai Nguyen University of Medicine and Pharmacy, Vietnam;

⁶Joint Vietnam-Russia Tropical Science and Technology Research Center, Vietnam

*Corresponding author: ntdat@ctctt.vast.vn or dhgiang@ctctt.vast.vn

(Received March 08^th, 2023)

Summary

Phytochemical Characterization and Bioactivity Evaluation of Ardisia sylvestris Leaves
from Lam Binh Mountainous District, Tuyen Quang Province

Ardisia sylvestris Pitard is a common herb which has been used widely for abdominal pain treatment. In this study, several chemical constituents, the antioxidant and anti-inflammatory activities of A. sylvestris leaves collected from the Lam Binh mountainous district, Tuyen Quang province, have been evaluated and compared to those of the samples from the domestic traditional medicinal market. The results indicated that the total phenolic contents of A. sylvestris samples from the Lam Binh district ranged from 12.61 to 18.96 mg GAE/g, while their total flavonoid contents varied between 7.85 and 13.27 mg QE/g. These were significantly higher than the contents of phenolic and flavonoid compounds of the marketed samples. Total alkaloid and tannin contents of A. sylvestris leaves collected from the Lam Binh were 1.39 - 3.94 mg/g and 2.54 - 3.94%, respectively, which were equal to those of the marketed samples. Moreover, extracts of A. sylvestris leaves from Lam Binh districts exhibited considerable DPPH radical-scavenging activity with IC₅₀ from 22.41 to 41.13 µg/mL, which were mostly higher than those of the positive control (ascorbic acid) and the market samples. Whereas, all samples demonstrated weak NO production inhibition without cytotoxicity in LPS-stimulated RAW264.7 macrophage cells. These were promising results for further investigation and cultivation of A. sylvestris in mountainous regions in the Lam Binh district as well as the other northern mountainous areas.

Keywords: Ardisia sylvestris, Antioxidant, Anti-inflammation.

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