Ấn phẩm

Potential Activities of Artemisia vulgaris Methanol Extract Against NTERA-2 Cancer Stem Cells (Tạp chí Dược liệu, tập 29, số 6/2024)

Journal of Medicinal Materials, 2024, Vol. 29, No. 6 (pp. 366 - 370)

 

POTENTIAL ACTIVITIES OF ARTEMISIA VULGARIS METHANOL EXTRACT AGAINST NTERA-2 CANCER STEM CELLS

Trieu Ha Phuong1,3, Tran Le Nam Khanh1, Nguyen Thi Cuc1, Nguyen Thi Nga1,3, Do Thi Phuong1, Le Hoang Ngan Ha1, Nguyen Thi Giang An4, Nguyen Trung Nam1, Do Thi Thao1,2,*

1Institute of Biotechnology, Vietnam Academy of Science and Technology

2Graduate University of Science and Technology, Vietnam Academy of Science and Technology

3University of Science and Technology of Ha Noi, Vietnam Academy of Science and Technology

4Faculty of Biology, College of Education, Vinh University

*Corresponding author: thaodo@ibt.ac.vn

(Received August 14th, 2024)

Summary

Potential Activities of Artemisia vulgaris Methanol Extract Against NTERA-2 Cancer Stem Cells

Cancer stem cells are the major cause of tumor initiation and metastasis, which can lead to drug resistance and recurrence in cancer patients. The search for natural chemicals that can inhibit the development of cancer stem cells is of interest to many scientists. Artemisia vulgaris L. (in Vietnamese named Ngai cuu) is known for traditional medicine to treat diseases such as diabetes, antiseptic, stomach aches, and cancer prevention. This study aims to evaluate the biological effect of methanol extract from A. vulgaris on the NTERA-2 cancer stem cell line. The activity of the extract was illustrated through cytotoxicity assay using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) method on monolayer and three-dimension cell culture models and in vitro migration assay. The extract of A. vulgaris exhibited inhibitory activity on NTERA-2 cells with an IC50 value of 18.31 µg/mL and reduced spheroid formation at the concentration of 500 µg/mL. Besides, this extract significantly reduced cell migration ability at the concentration of IC50 and 2xIC50. Consequently, A. vulgaris crude methanol extract presented obvious effects on NTERA-2 cancer stem cells, providing a new direction for investigating natural compound ingredients of this plant for more efficient cancer treatment.

Keywords: Artemisia vulgaris, NTERA-2, Cytotoxicity, Tumorsphere formation assay, Scratch assay.

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