Journal of Medicinal Materials, 2020, Vol. 25, No. 3 (pp. 180 - 185)
PREPARATIVE ENRICHMENT OF SAPONINS FROM FRUITS
OF MOMORDICA CHARANTIA
BY D101 MACROPOROUS ADSORPTION RESINS
Nguyen Quoc Tuan*, Dinh Thi Quynh Anh, Pham Quoc Tuan*
Centre for Drug Research and Technology Transfer - Phu Tho College of Medicine and Pharmacy, Vietnam
*Corresponding author: quoctuan3012@naver.com or phamquoctuan@duocphutho.edu.vn
(Received May 20th, 2020)
Summary
Preparative Enrichment of Saponins from Fruits of Momordica charantia by D101 Macroporous Adsorption Resins
Saponins are active compounds in natural products. Many researchers have tried to find the method to determine their content in herbs. To establish a simple extraction method for the enrichment of saponins from the fruits of Momordica charantia, we used D101 macroporous adsorption resins with optimal conditions in the concentration of loading sample is 6 mg/mL (based on the weight of the crude drug) and the volume of loading sample is 40 BV (bed volume). The extract was passed through the D101 resin column at a flow rate of 5 BV/h. After adsorption, the adsorbed column was first washed with 4 BV of distilled water, and then desorbed by 80% ethanol at a flow rate of 3 BV/h. The contents of saponin in the product were improved greatly after the separation procedure, by up to 15.20 ± 0.47 %.
Keywords: Momordica charantia, Saponin, Macroporous adsorption resins, D101.
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