Journal of Medicinal Materials, 2019, Vol. 24, No. 3 (pp.131 - 138)
QUALITY EVALUATION OF FALLOPIA MULTIFLORA IN VIETNAM BASED ON HPLC-FLD AND CHEMOMETRICS
Nguyen Thi Ha Ly1,2,*, Ta Thi Thao2, Phuong Thien Thuong1
1National Institute of Medicinal Materials (NIMM), Hanoi, Vietnam;
2Faculty of Chemistry, VNU University of Science, Vietnam National University, Hanoi, Vietnam
*Corresponding author: firstname.lastname@example.org
(Received July, 18th, 2018)
Quality Evaluation of Fallopia multiflora in Vietnam Based on HPLC-FLD and Chemometrics
In this study, two simple and sensitive HPLC methods with fluorescence detector (FLD) were developed and validated for quantitation of three stilbenes (2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (1), resveratrol (2), piceid (3)) and two anthraquinones (emodin (4), physcion (5)) in Radix Fallopiae multiflorae. The separation was carried out on column Ascentis C18 (250 mm x 4.6 mm; 5 µm). All calibration curves showed good linearity (R2> 0.999) within test ranges. The overall recovery was 95.0 - 106.0%. RSD for intra- and inter-day repeatability of peak areas of 5 analytes were less than 4%. The proposed methods have high sensitivity with the LODs of 5 analytes (1-5) were 0.3 µg/mL, 0.4 µg/mL, 0.4 µg/mL, 4.9 µg/mL, 3.42 µg/mL, respectively. The validated methods were successfully applied for quantification of 5 compounds in samples of Radix Fallopiae multiflorae from different locations of Vietnam. Chemometrics such as principal component analysis (PCA) and clustering analysis (CA) were used to evaluate homogeneity of Radix Fallopiae multiflorae in Vietnam, which suggested that their quality homogeneity was good.
Keywords: Radix Fallopiae multiflorae, Stilbene, Anthraquinone, RP-HPLC-FLD, Chemometric.
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