Journal of Medicinal Materials, 2023, Vol. 28, No. 4 (pp. 223 - 229)

QUANTITATIVE ANALYSIS OF TOTAL POLYPHENOLS IN THE LEAVES OF TERMINALIA CATAPPA L. BY UV-VIS ABSORPTION SPECTROSCOPY

Ha Van Oanh^a, Chu Thi Thanh Huyen^a, Nguyen Manh Tuyen, Nguyen Tien Dung, Do Quyen*

Ha Noi University of Pharmacy, Vietnam;

^aThese authors contributed equally to this manuscript as joint first authors

*Corresponding author: doquyen@hup.edu.vn

(Received July 19^th, 2023)

Summary

Quantitative Analysis of total Polyphenols in the Leaves of Terminalia catappa L. by UV-Vis Absorption Spectroscopy

An UV-Vis absorption spectroscopy method had been developed and validated to quantify total polyphenols in the leaves of Terminalia catappa L. (TC). The TC leaf powder was extracted by ultrasonic method with 50% ethanol, followed by the reaction with Folin-Ciocalteu phenol reagent and photometric measurement at 730 nm. The developed method exhibited good linearity (R² = 0.9957) within a range of 1-8 µg/ml and LOD and LOQ were found to be 0.033 and 0.100 µg/ml, respectively. The developed method was also found accurate, and precise according to the regulatory guidelines. The developed method was applied to measure the total polyphenol content in samples collected from various provinces in Vietnam.

Keywords: Terminalia catappa, Total polyphenols, UV-Vis.

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