Journal of Medicinal Materials, 2023, Vol. 28, No. 2 (pp. 103 -109)

QUANTITATIVE DETERMINATION OF MIMOSINE

IN THE MEDICINAL HERB MIMOSA PUDICA MIMOSACEAE BY HPLC-PDA

Nguyen Thi Thanh Van¹, Vo Quoc Ngu², Huynh Lam Diem My², Duong Hong To Quyen³,

Phan Van Ho Nam¹, Hua Hoang Oanh¹,*

¹University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam;

 ²University of Natural Sciences, Vietnam National University, Ho Chi Minh City, Vietnam;

 ³Hospital of Traditional Medicine, Ho Chi Minh City, Vietnam

*Corresponding author: hhoanh@ump.edu.vn

(Received February 04^th, 2023)

Summary

Quantitative Determination of Mimosine in the Medicinal Herb Mimosa pudica Mimosaceae by HPLC-PDA

An HPLC method was proposed for quantification of mimosine in the aerial parts of Mimosa pudica. The chromatography was established as: using the C₁₈ (250 mm × 4.6 mm, 5 µm) column; the PDA detector was set at 275 nm; the mobile phase consisted of methanol and pH 2.0 buffer with ion-pairing agent sodium hexane-1-sulfonate, gradient elution; flow rate was 1 mL/min; the column temperature was set at 35°C; injection volume was 20 µL. The method was validated for the system suitability (RSD of peak area = 1.18%; RSD of t_R = 0.03%); specificity; linearity ranging from 0.5 - 20.0 µg/mL (R² = 1), precision (RSD < 3.0%) and accuracy (recovery: 92.43% - 98.11%). As for practical application, the tested samples showed the content of mimosine ranging from 64.5 µg/g to 363.1 µg/g, on dried basis.

Keywords: Mimosine, Mimosa pudica, Specification, HPLC-PDA.

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