Journal of Medicinal Materials, 2022, Vol. 27, No. 1 (pp. 51 - 56)

THE OPTIMAL MANUFACTURE OF POLYSACCHARIDES FROM SOYBEAN AS STABILIZATION OF OIL-IN-WATER EMULSION

Ngo Thi Lan Huong¹, Dao Anh Hoang¹, Nguyen Van Khanh², Nguyen Thi Ly¹,

Nguyen Thi Le¹, Hoang Le Son^1,*

¹National Institute of Medicinal Materials, Hanoi, Vietnam;

²University of medicine and pharmacy, Vietnam National University

*Corresponding author: hoangson.med@gmail.com

(Received September 25^th, 2021)

Summary

The Optimal Manufacture of Polysaccharides from Soybean as Stabilization of Oil-in-Water Emulsion

The Resolution IV Factorial design (RIVF) and response surface methodology (RSM) combination with desirability function (DF) techniques were integrated to estimate optimal manufacture of the soybean polysaccharide that lead to maximizing the yields (Y₁) and content of polysaccharide (Y₂) and minimizing the value of emulsifying activity index -EAI (Y₃). The RIVF was used to determine the variable factors (X₁-X₃) as well as the fixed factor (X₄-X₆). The three mathematical models were developed by using RSM with experimental results. The DF was used to optimize the mathematical model and predict the optimal value of factors. The optimal parameters of manufacture were the extraction two time in 2.5h (X₂) with ratio of solid to water pH 6 (X₄) as 1:10 (X₃) at temperature 90^oC (X₅) and the purification with the ratio water to ethanol 87% (X₁) as 1:5 (X₆). The manufacture also was confirmed through experiments. This polysaccharide could be the good candidates of excipients to stabilize oil-in-water emulsion.

Keywords: Soybean, polysaccharides, Emulsifying activity index, EAI, Desirability function.

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