Antioxidant and Hepatoprotective Activity of
Polygonum tomentosum Willd. in vitro and on Damaged Liver Mice Induced by Carbon Tetrachloride
Polygonum tomentosum Willd. is a perennial herb growing in marshy areas, roadsides, along ditches and field margins of Vietnam. Preliminary study revealed that the ethanol extract possesses a strong antioxidant property. In this study, the ethanol extract of the aerial parts of the plant was fractionated into chloroform (CHCl3-F), ethyl acetate (Et-F), n-butanol (Bu-F) and aqueous (A-F) fractions, according to their polarity and solubility. The antioxidant activity of fractions were determined by FRAP, DPPH assays, and hepatoprotective activity on ex vivo test with mouse liver cells and CCl4 intoxicated mice. The results showed that Et-F had higher antioxidant and hepatoprotective activities than those of the other fractions. The result of DPPH• radical scavenging assay revealed that the Et-F (IC50 = 2.78 mg/ml), had a better in vitro antioxidant activity than the pure natural antioxidant ascorbic acid (IC50 = 3.24 mg/ml). Ex vivo and in vivo hepatoprotective activity of Et-F on injury mouse liver induced by carbon tetrachloride was comparable to Silymarin. The results obtained in the present study demonstrated that P. tomentosum is a potential source of natural antioxidants and hepatoprotective agent.
Keywords: Polygonum tomentosum, antioxidant activity, FRAP, DPPH, CCl4, hepatoprotective effect
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