Enzymatic procedure for isolation of chitin from shrimp shells

The procedure for isolation of chitin using proteolytic activity of bromelanin extracted from pine-apple waste was proposed. This enzymatic-processing procedure was successfully used for isolation of chitin from shrimp shells. The enzymatic method included following steps: (1) treating shrimp shells with the extracts from pine-apple waste (for removal of proteins and minerals), (2) further removal of protein by 2% alkali (NaOH), and (3) removal of pigments by KMnO4 and oxalic acid.  This enzymatic-processing procedure is thought to be more useful than a well known routine chemical procedure for isolation of chitin from shrimp shells.

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