Journal of Medicinal Materials, 2024, Vol. 29, No. 2 (pp. 125 - 128)
CORN STEEP LIQUOR PREPARATION
FOR USE IN THE FERMENTATION PROCESS
Le Ngoc Khanh1, Dam Thanh Xuan1, Bui Thi Thuy Luyen2, Vu Thanh Thao3, Nguyen Khac Tiep1,*
1Faculty of Biotechnology, Hanoi University of Pharmacy, Vietnam;
2Faculty of Pharmaceutical Chemistry and Technology, Hanoi University of Pharmacy, Vietnam;
3Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
*Corresponding author: tiepnk@hup.edu.vn
(Received March 15th, 2024)
Summary
Corn Steep Liquor Preparation for Use in the Fermentation Process
The primary goal of this work was to develop a laboratory-scale process for producing corn-steep liquor from locally available materials to replace imported commercial products used in microbe culturing media. Additionally, the study aimed to compare the quality of our locally-made corn-steep liquor with commercial products. The findings indicated that corn sourced from Hai Duong Province had more advantages as a starting material for production. The corn-steep liquor was obtained using the process of hot water extraction, wherein 2 kilograms of corn kernel were soaked in water at a temperature of 50°C for 48 hours (water-to-corn ratio of 1.5:1). The resulting liquid was separated and condensed after the extraction process. The locally-produced corn-steep liquor meets the required moisture, pH, and total amino acid content standards, surpassing the imported products in protein concentration. The obtained corn-steep liquor was used for calcium lactate and antibiotics fermentation process, exhibiting a comparable overall yield to commercially available products. The potential use of our corn-steep liquor to substitute imported products in industrial fermentation applications is worth considering.
Keywords: Corn-steep liquor, Extraction, Amino acid, Total nitrogen, Microorganism media.
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