Ấn phẩm

1H and 13C NMR Assignments of Phenolic and other Constituents from Miliusa sinensis Leaves and their Cytotoxic Activity (Tạp chí dược liệu, tập 27, tập 27, số 6/2022)

Journal of Medicinal Materials, 2022, Vol. 27, No. 6 (pp. 336 - 343)

 

1H AND 13C NMR ASSIGNMENTS OF PHENOLIC

AND OTHER CONSTITUENTS FROM MILIUSA SINENSIS LEAVES

AND THEIR CYTOTOXIC ACTIVITY

Cao Thi Hue1, Pham Thanh Binh2, Luc Quang Tan3, Hoang Van Hung3, Vu Thanh Trung2, Nguyen Thi Lan Huong1, Nguyen Manh Ha4, Nguyen Van Thanh2, Nguyen Phuong Thao2,*

1Thuyloi University, Hanoi, Vietnam;

2Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam;

3Thai Nguyen University-Lao Cai Campus, Lao Cai Province, Vietnam;

4University of Mining and Geology, Hanoi, Vietnam

*Corresponding author: thaonp@imbc.vast.vn

(Received August 31st, 2022)

Summary

1H and 13C NMR Assignments of Phenolic and other Constituents from Miliusa sinensis Leaves
and their Cytotoxic Activity

Phytochemical investigation of a methanolic extract of Miliusa sinensis leaves led to the isolation of eight compounds (18), using various chromatographic separations. Their structures were elucidated to be pteleifoside C (1), melisimplexin (2), 3,5,6,7,3',4'-hexamethoxyflavone (3), 6'-O-β-D-apiofuranosylthalictoside (4), osmanthuside H (5), icariside D1 (6), 3,4-dimethoxyphenol 1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (7), and 3,4,5-trimethoxyphenol 1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (8) by detailed analysis via spectroscopic techniques (1D, 2D NMR, and MS data) as well as comparison with those reported. All of the isolates were evaluated for their cytotoxicity against HepG2, DU145, A549, and MCF7 cancer cell lines, using the MTT assay. Compounds 1-8 showed weak or fewer effects against the tested cancer cell lines. This is the first time that glycoside constituents from M. sinensis leaves and their cytotoxic activity were reported.

Keywords: Miliusa sinensis; Annonaceae; Flavonoid; Megastigmane; Phenolic; Cytotoxic activity.

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