Journal of Medicinal Materials, 2021, Vol. 26, No. 1+2 (pp. 34 - 39)

A NEW COMPOUND, 1b,3b,12b,20(S)-TETRAHYDROXYDAMMAR-24-ENE, ISOLATED FROM GYNOSTEMMA COMPRESSUM AND ITS AMPK

AND ACC ACTIVATION IN 3T3-L1 ADIPOCYTES

Dinh Thi Thanh Thuy^1,2, Nguyen Thi Thu¹, Ngo Huy Trung¹,

Tran Thi Hien⁴, Pham Thanh Huyen¹, Pham Thanh Ky^3,*, Do Thi Ha^1,*

¹National Institute of Medicinal Materials (NIMM), Hanoi, Vietnam;

²National Hospital for Tropical Diseases, Hanoi, Vietnam;

³Hanoi University of Pharmacy, Hanoi, Vietnam;

⁴Thai Binh University Medicine and Pharmacy, Thai Binh, Vietnam

*Co-corresponding authors: hado.nimms@gmail.com

(Received February 04^th, 2021)

Summary

A New Compound, 1b,3b,12b,20(s)-Tetrahydroxydammar-24-ene, Isolated from Gynostemma compressum

and its AMPK and ACC Activation in 3T3-L1 Adipocytes

A new dammarane saponin, 1β,3β,12β,20(S)-tetrahydroxydammar-24-ene (1) together with three known compounds, including corchoionol C (2), 5-hydroxymethylfurfural (3), and gycomoside II (4) were isolated from the 80% ethanol extract of Gynostemma compressum X. X. Chen & D. R. Liang. Their chemical structures were determined by using extensive NMR data, mass spectroscopy, and comparing with published literature. The ability of the new compound to phosphorylate AMPK and ACC in 3T3-L1 cells was evaluated. Compound 1 (10 μM) upregulated p-ACC expression and induced the most significant change in p-AMPK expression. These results suggested that the new compound has potential effects on glucose and lipid metabolism.

Keywords: Gynostemma compressum, Dammarane saponin, AMPK, ACC, 3T3-L1.

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