Journal of Medicinal Materials, 2019, Vol. 24, No. 3 (pp. 174 - 180)
ANTIPROLIFERATIVE EFFECTS OF BETULINIC ACID
AND FLAVONOIDS FROM LICORICE WEED (SCOPARIA DULCIS L.)
ON HUMAN CANCER CELL LINES
Tran Thi Van Anh1, Tran Thi Phuong Uyen2, Ly Kien Hoa1, Do Thi Hong Tuoi1,*
1Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City;
2Faculty of Pharmacy, Nguyen Tat Thanh University
*Corresponding author: hongtuoi@ump.edu.vn
(Received May, 12th, 2019)
Summary
Antiproliferative Effects of Betulinic Acid and Flavonoids from Licorice Weed (Scoparia dulcis L.) on Human Cancer Cell Lines
Betulinic acid (1) and four flavonoids including hispidulin (2), apigenin (3), pectolinagenin-7-O-rutinoside (4), hispidulin-7-O-rutinoside (5) isolated from licorice weed (Scoparia dulcis L.) were tested for their antiproliferative activity on three human cancer cell lines and on one normal cell line using MTT test. All tested compounds showed no antiproliferative effect against the pig kidney normal cells (LLC-PK1) and the liver cancer cells (HepG2) at the range of concentrations from 4 to 100 μM. Compounds 1, 2, 3 exhibited the cytotoxic activity in the breast cancer cells (MDA-MB-231) with IC50 of 17.6 µM, 86.6 µM and 13.4 µM, respectively. Compounds 1 and 3 decreased the growth of the muscle rhabdomyosarcoma cells (RD) with IC50 of 2.94 µM and 42.5 µM, respectively. The mechanism of antiproliferative effect of compound 1 was further examined by the LDH (lactate dehydrogenase) release assay and the detection of apoptosis.
Keywords: Scoparia dulcis L., Flavonoid, Betulinic acid, Antiproliferative effect.
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