Journal of Medicinal Materials, 2022, Vol. 27, No. 2 (pp. 107 - 111)
DEVELOPMENT AND VALIDATION OF FLAME ATOMIC ABSORPTION SPECTROMETRY (FAAS) FOR THE DETERMINATION OF POTASSIUM IN STACHYS AFFINIS ROOTS
Tran Thi Thu Hien*, Nguyen Thi Van Anh, Vo Thi Bach Hue
Faculty of Pharmacy, Lac Hong University, Dong Nai Province, Vietnam.
(Received March 29th, 2022)
Development and Validation of Flame Atomic Absorption Spectrometry (FAAS)
for the Determination of Potassium in Stachys affinis Roots
Stachys affinis was considered a food and a medicinal plant with the s of anti-oxidation, antibacteria, anti-inflammation... and contains high content of potassium in roots. In this study, for development of potassium in Stachys affinis, Box-Behnken model was applied to optimize the sample digestion followed by FAAS. The experimental variables chosen were ashing time (270 - 390 min), furnace temperature (450 - 650°C), and nitric acid concentration (16 - 64%). Concentration values of potassium were used as a response. The potassium concentration can reach as high as 17.74 ± 0.26 mg/g at the ashing time of 340 min, furnace temperature of 461°C, and nitric acid concentration 38%. The analytical method developed and validated according to ICH and AOAC guidelines met the requirements of selectivity, precision ( RSD = 1.44%), accuracy (recovery rate ranged from 95.6% to 107.8%). The calibration curve was obtained using linear regression y = 0.1049x - 0.0102 (R2 = 0.9991) in the range of potassium concentration from 0.2 ppm - 5 ppm. The LOD and LOQ values for potassium were 0.046 ppm and 0.138 ppm, respectively.
Keywords: Box-Behnken design, FAAS, Stachys affinis.
(Nguồn tin: )