Journal of Medicinal Materials, 2022, Vol. 27, No. 4 (pp. 222 - 227)
DEVELOPMENT OF STACHYOSE QUANTITATIVE DETERMINATION
IN STACHYS AFFINIS BUNGE BY LC - MS AND ITS APPLICATION
FOR ESTABLISHMENT OF STACHYOSE REFERENCE STANDARD
Nguyen Thi Bich Ngoc1,2, Dang Thi Xuan Quyen3, Nguyen Thi Vy Phuong3, Vo Thi Bach Hue4, Phan Nguyen Truong Thang5, Phan Van Ho Nam1,*
1Department of Analytical Chemistry and Quality Control of Drug, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam;
2NTT High Technology Institute - Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam;
3Tipharco Pharmaceutical Joint Stock Company;
4Department of Analytical Chemistry and Quality Control of Drug, Lac Hong University,
Dong Nai Province, Vietnam;
5Institute of Drug Quality Control Ho Chi Minh City, Vietnam.
*Corresponding author: firstname.lastname@example.org
(Received March 27th, 2022)
Development of Stachyose Quantitative Determination in Stachys affinis Bunge by LC - MS
and its Application for Establishment of Stachyose Reference Standard
Stachyose is the main ingredient in Stachys affinis rhizomes and has many pharmacological effects such as lowering blood sugar, strengthening the immune system, and relieving constipation, etc. To monitor the quality of the new material and its products, the quantitative determination of stachyose in Stachys affinis Bunge rhizomes was developed according to the guidelines of ICH (2005) and AOAC (2012). Besides, the established procedure was applied to determine the purity of stachyose as following requirements of a reference standard.
The procedure was UPLC-MS system (ACQUITY QDa Waters); Phenomenex Gemini NX-C18 (4.6 ⨯ 150 mm; 5 µm) column; acid formic 0.05% and MeOH as mobile phase in isocratic mode; flow rate of 0.5 mL/min; detector MS/ESI- (665 m/z); cone voltage 40 V; capillary voltage 0.8 kV. Standards and test samples are all diluted in water before analysis.
Keywords: Stachys affinis Bunge, Stachyose, Reference standard, LC-MS.
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