Journal of Medicinal Materials, 2017, Vol. 22, No. 5 (pp. 293 - 297)

EFFECT OF AQUEOUS AND ETHANOL EXTRACTS FROM AN GIANG TRADITONAL REMEDY ON OLEIC ACID-INDUCED LIPID ACCUMULATION IN HEPATIC CELLS

Pham Thi Nguyet Hang^1,*, Le Viet Dung¹, Duong Mong Ngoc²

1. National Institute of Medicinal Materials, Hanoi, Vietnam (NIMM);
2. Research Center of Ginseng and Medicinal Materials (NIMM)

*Corresponding author: pnhang2004@yahoo.com

(Received August, 23^rd, 2017)

Summary

Effect of Aqueous and Ethanol Extracts from An Giang Traditonal Remedy on Oleic Acid-Induced Lipid Accumulation in Hepatic Cells

The aim of this study is to evaluate the effects of ethanol and aqueous extracts of an An Giang traditional remedy in reducing lipid accumulation in hepatic cells (HepG2). HepG2 cells were treated with oleic acid with or without an aqueous or an ethanol extract of an An Giang traditional remedy to observe the lipid accumulation by Oil Red O stain. Fenofibrate was used as a positive control. We used Oil Red O dye to quantify the accumulation of lipids in the cell. The results showed that only the ethanol extract at concentration 25 μg/ml and 50 μg/ml suppressed oleic acid-induced lipid accumulation in HepG2 cells with the inhibition rate of 9.40 ± 1.96 and 16.05 ± 1.40% respectively. The results suggested that the ethanol extract of an An Giang traditional remedy inhibited lipid accumulation in hepatic cells.

Keywords: An Giang Traditional Remedy, HepG2 cells, Inhibited lipid accumulation, Oil red O staining.

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