Journal of Medicinal Materials, 2017, Vol. 22, No. 3 (pp. 306 - 310)
HPTLC QUANTITATIVE ANALYSIS OF TWO STILBENOIDS IN RADIX FALLOPIAE MULTIFLORAE COLLECTED FROM VARIOUS GEOGRAPHICAL REGIONS OF VIETNAM
Nguyen Thi Ha Ly1,2,*, Phuong Thien Thuong1, Ta Thi Thao2, Nguyen Thi Phuong1
1National Institute of Medicinal Materials (NIMM), Hanoi, Vietnam
2Faculty of Chemistry, Natural sciences University, Vietnam
*Corresponding author: email@example.com
(Received July, 18th, 2017)
HPTLC Quantitative Analysis of two Stilbenoids in Radix Fallopiae multiflorae Collected from Various Geographical Regions of Vietnam
A simple, sensitive and precise high performance thin-layer chromatographic (HPTLC) method for analysis of two stilbenes: 2,3,5,4՛-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) and resveratrol (RES) in Radix Fallopiae multiflorae (RFM) was developed and validated. The separation was achieved on TLC aluminum plates pre-coated with silica gel 60 F254 using toluene-ethyl acetate –acetone - formic acid (5:2:2:1, v/v/v/v) as a mobile phase. Densitometric analysis was performed at 320 nm. This system was found to have compact spot of THSG and RES at Rf values of 0.23 and 0.57, respectively. For the proposed procedure, linearity (R2= 0.994 for THSG, R2= 0.998 for RES), limit of detection (3.2 ng/spot for THSG and 4.0 ng/spot for RES), limit of quantification (10.5 ng/spot for THSG and 13.2 ng/spot for RES), recovery (ranging from 97.92% - 99.93% for THSG and 95.55% - 100.93% for RES) were found to be satisfactory. Statistical analysis revealed that the content of THSG and RES in RFM collected from various geographical regions in Vietnam varied significantly. The highest concentration of THSG and RES in RFM was found to be present in samples collected from Ha Giang (North-east regions of Vietnam).
Keywords: HPTLC, Validation, Radix Fallopiae multiflorae, Stilbene.
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