Journal of Medicinal Materials, 2017, Vol. 22, No. 3 (pp.162-166)
IN VITRO ACETYLCHOLINESTERASE INHIBITION AND FREE RADICAL SCAVENGING ACTIVITY OF ACANTHOPANAX GRACILISTYLUS W.W. SMITH EXTRACT
OLAPOJU Samuel O.1, Pham Thi Nguyet Hang2,*
*Corresponding author: email@example.com
(Received April, 07th, 2017)
In vitro Acetylcholinesterase Inhibition and Free Radical Scavenging Activity of Acanthopanax gracilistylus W.W. Smith Extract
This research was designed to evaluate the in vitro acetylcholinesterase (AChE) inhibitory ability and antioxidant effect of Acanthopanax gracilistilylus W.W. Smith (AG) ethanol extract. The effect of AG extract on AChE was evaluated by Ellman method and antioxidant effect of AG was investigated by using DPPH and superoxide scavenging in vitro assay. The results from the AChE inhibition showed that AG extract has the ability to inhibit AChE up to 79.80% at concentration 3.0 mg/ml with an IC50 value of 1.50 mg/ml. The in vitro DPPH free radical scavenging activity of AG extract was found to be up to 88.67% at concentration 250 µg/ml with an IC50 value of 88.90 µg/ml. AG extract also showed a weak superoxide scavenging effect and at 3.00 mg/ml, 57.59% of superoxide was inhibited with IC50 found to be 2.70 mg/ml.
Keywords: Acanthopanax gracilistilylus ethanol extract, Acetylcholinesterase inhibition, Radical scavenging, DPPH, Superoxide.
(Nguồn tin: Tạp chí Dược liệu - Viện Dược liệu)