Journal of Medicinal Materials, 2020, Vol. 25, No. 5 (pp. 311 - 316)
IN VITRO AND IN VIVO INHIBITORY EFFECTS ON THE HMG-COA REDUCTASE OF OKRA EXTRACTS (ABELMOSCHUS ESCULENTUS (L.))
Vuong To Uyen, Nguyen Thi Thuy Anh, Nguyen Ngoc Phuc, Huynh Ngoc Trinh*
Department of Pharmacology, Faculty of Pharmacy, University of Medicine and Pharmacy
at Ho Chi Minh City, Vietnam
*Corresponding author: email@example.com
(Received August, 27th, 2020)
In vitro and In vivo Inhibitory Effects on the HMG-CoA Reductase of Okra Extracts (Abelmoschus esculentus (L.))
Previous studies have clearly showed the hypolipidemic effect of total extract and ethyl acetate extract from aerial parts of Okra (Abelmoschus esculentus (L.), Malvaceae) in experimental mice. This study aimed to elucidate the mechanism of hypolipidemic effect of these extracts by inhibiting HMG-CoA reductase enzyme. The in vitro experiment was conducted on the HMG-CoA reductase isolated from healthy mouse liver to determine IC50 of total extract and ethyl acetate extract compared with the reference drug atorvastatin. The in vivo experiment was performed on the Triton WR-1339 induced hyperlipidemia in mice. The treatment of Okra extracts was enrolled by oral administration at the doses equivalent to 60g of dry powder/kg body weight. The in vitro IC50 values of total extract and ethyl acetate extract and atorvastatin on HMG-CoA reductase were 1,002.03 μg/ml, 445.97 μg/ml and 6.89 ng/ml, respectively. In in vivo experiment, the serum total cholesterol and triglycerides levels of treated groups were significantly lower than these of untreated group. Moreover, these treatments also inhibited hepatic HMG-CoA reductase activity. However, the HMG-CoA reductase activity of groups treated with total extract and ethyl acetate extract were higher than physiological group. Thus, the total extract and ethyl acetate extract significantly showed hypolipidemic effect by inhibiting HMG-CoA reductase activity.
Keywords: HMG-CoA reductase, Okra, Triton WR-1339.
(Nguồn tin: )